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The feed was produced by Nofima Feed Technological know-how Centre in Bergen and shipped to Sunndalsøra ahead of the beginning of the examine. The basal food plan consisted of 50 % fishmeal and ten % Every of wheat, wheat gluten and soya food focus, As well as minerals and vitamin mixture. A detailed description of all feed substances is introduced in on the web Supplementary Table S3. 5 distinctive experimental meal plans ended up tested, with triplicate tanks for each diet plan team and one hundred fish for every tank.
Mind, skin, eye, liver and heart samples had been subsequently excised and frozen in liquid N2 right before storing the samples at −80°C until finally afterwards Examination of lipid composition. Hence, a few pooled samples of 10 particular person organ/tissue samples (ten fish from each tank, thirty fish in overall) have been analysed per food plan group.
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